@article {Perez-Feito:February 2006:1061-4303:181,
	author = "Perez-Feito, Rafael",
	author = "Peccia, Jordan",
	author = "Noguera, Daniel R",
	title = "Comparison between Direct Microscopy and Flow Cytometry for rRNA-Based Quantification of Candidatus Accumulibacter phosphatis in Activated Sludge",
	journal = "Water Environment Research",
	volume = "78",
	year = "February 2006",
	abstract = "A comparison of the quantification of a specific microbial group in activated sludge by fluorescent in-situ hybridization, coupled with either direct microscopic counting or flow cytometry, was performed using an enhanced-biological-phosphorus-removal, sequencing-batch reactor. The population dynamics of Candidatus Accumulibacter phosphatis ( <I>Cand.</I> A. phosphatis) was evaluated during two separate runs of the reactor. With the operational conditions used, <I>Cand.</I> A. phosphatis was enriched until a failure in the pH controller eliminated its ecological advantage. As a result, the comparison of quantification techniques included <I>Cand.</I> A. phosphatis concentrations as low as 11&#37; and as high as 96&#37; of the total cells in the samples. The analysis demonstrated that, regardless of the particular limitations of each technique, both provided similar results when the activated-sludge flocs were easily dispersed. However, when the activated-sludge samples contained flocs that were difficult to disperse, flow cytometry failed to provide quantitative results.",
	pages = "181-188(8)",
	url = "http://www.ingentaconnect.com/content/wef/wer/2006/00000078/00000002/art00010"
	doi = "doi:10.2175/106143005X89634"
}