@article {Sanchez-Cortes:January 2006:0003-7028:48, author = "Sanchez-Cortes, S.", author = "Corrado, G.", author = "Trubetskaya, O.E.", author = "Trubetskoj, O.A.", author = "Hermosin, B.", author = "Saiz-Jimenez, C.", title = "Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion ChromatographyPolyacrylamide Gel Electrophoresis (SEC-PAGE)", journal = "Applied Spectroscopy", volume = "60", year = "January 2006", abstract = "A humic acid extracted from a chernozem soil was fractionated combining size exclusion chromatography and polyacrylamide electrophoresis (SEC-PAGE). Three fractions named A, B, and C+D, with different electrophoretic mobilities and molecular sizes (MS), were obtained and subsequently characterized by thermochemolysis and surface-enhanced Raman spectroscopy (SERS). The data confirmed that fraction A, with the higher MS, was more aliphatic than fractions B and C+D and, in turn, fractions with lower MS (B and C+D) denoted an enrichment in lignin residues. These structural features explain conformational changes when varying the pH in the humic fraction A and indicated that combination of the two techniques is a good approach for characterizing humic substances.", pages = "48-53(6)", url = "http://www.ingentaconnect.com/content/sas/sas/2006/00000060/00000001/art00012" doi = "doi:10.1366/000370206775382695" }