@article {Hanley:1 February 2002:0003-7028:155,
	author = "Hanley, Quentin S.",
	author = "Arndt-Jovin, Donna J.",
	author = "Jovin, Thomas M.",
	title = "Spectrally Resolved Fluorescence Lifetime Imaging Microscopy",
	journal = "Applied Spectroscopy",
	volume = "56",
	year = "1 February 2002",
	abstract = "We report a system for collecting spectrally resolved fluorescent lifetime images. Frequency domain fluorescence lifetime detection was combined with two-dimensional spectral imaging in a programmable array microscope. The spectroscopic fluorescence lifetime imaging microscopy (sFLIM) system has a resolution of ~50 (&#955;/&#916;&#955;) in the current arrangement and a wavelength range of ~430-750 nm. With the sFLIM system, we recorded the lifetime spectra of rhodamine 6G, rhodamine B, and the DNA intercalation dye propidium iodide (PI) in cuvettes and an EGFP-fusion of the histone 2A variant D protein in <I>Drosophila</I> salivary gland explants in the presence and absence of PI. In the absence of PI, the EGFP-fusion exhibited a lifetime of 2.7 ns with little variation in wavelength. The lifetime of PI alone ranged from ~1 ns in buffer to ~18 ns when intercalated in the nuclei of intact cells. The combination of EGFP and PI in the <I>Drosophila</I> salivary gland explants exhibited strong fluorescence resonance energy transfer (FRET), a result consistent with the known nucleosomal structure of eukaryotic chromatin.",
	pages = "155-166(12)",
	url = "http://www.ingentaconnect.com/content/sas/sas/2002/00000056/00000002/art00003"
	doi = "doi:10.1366/0003702021954610"
}