@article {Singh:1 August 2000:0022-3573:991,
	author = "Singh R.P.",
	author = "Banerjee S.",
	author = "Ramesha Rao A.",
	title = "Effect of Aegle marmelos on Biotransformation Enzyme Systems and Protection Against Free-radical-mediated Damage in Mice",
	journal = "Journal of Pharmacy and Pharmacology",
	volume = "52",
	year = "1 August 2000",
	abstract = "The effect of hydroalcoholic (80&#037; ethanol, 20&#037; water) extract of leaves of <I>Aegle marmelos</I> was examined on carcinogen-metabolizing phase-I and phase-II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase and lipid peroxidation, using two doses of dried extract (50 and 100&#160;mg&#160;kg<SUP>-1</SUP> daily for 14 days), in the liver of mice. The modulatory effect of the extract was also examined on extrahepatic organs (lung, kidney and fore-stomach) for effects on the activity of glutathione S-transferase, DT-diaphorase, superoxide dismutase and catalase.<P></P>Extract treatment significantly increased the basal levels of acid-soluble sulphydryl (&#150;SH) content, cytochrome P450, NADPH-cytochrome P450 reductase, cytochrome b5, NADH-cytochrome b5 reductase, glutathione S-transferase, DT-diaphorase, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in the liver. <I>Aegle</I> acted as a bifunctional inducer since it induced both phase-I and phase-II enzyme systems. Both doses significantly decreased the activity of lactate dehydrogenase and formation of malondialdehyde in liver, suggesting a role in cytoprotection as well as protection against pro-oxidant-induced membrane damage. Butylated hydroxyanisole (positive control) induced almost all the antioxidative parameters measured in this study. The extract was effective in inducing glutathione S-transferase, DT-diaphorase, superoxide dismutase and catalase in lung, glutathione S-transferase, DT-diaphorase and superoxide dismutase in fore-stomach, and DT-diaphorase and superoxide dismutase in lung.<P></P>These significant changes in the levels of drug-metabolizing enzymes and antioxidative profiles are strongly indicative of the chemopreventive potential of this plant, especially against chemical carcinogenesis.",
	pages = "991-1000(10)",
	url = "http://www.ingentaconnect.com/content/rpsgb/jpp/2000/00000052/00000008/art00012"
	doi = "doi:10.1211/0022357001774714"
}