IngentaConnect Effect of Lead Acetate on Cytosolic Thioredoxin Reductase Activit...

Authors: Conterato, Greicy M. M.¹; Augusti, Paula R.¹; Somacal, Sabrina²; Einsfeld, Lídia²; Sobieski, Rocheli²; Torres, Juliana R.V.²; Emanuelli, Tatiana²

Source: Basic & Clinical Pharmacology & Toxicology, Volume 101, Number 2, August 2007 , pp. 96-100(5)

Publisher: Blackwell Publishing

Abstract:

Oxidative stress has been suggested to be an important molecular mechanism of toxic effects of lead in the kidney. Thioredoxin reductase-1 is a selenoprotein involved in many cellular redox processes. This study evaluated the effect of acute and chronic exposure intraperitoneally to lead acetate on thioredoxin reductase-1 activity and on other oxidative stress parameters in the rat kidney, as well as on indicators of renal function commonly used to assess lead poisoning. Acute exposure to 25 mg/kg lead acetate increased superoxide dismutase and thioredoxin reductase-1 activity (after 6, 24 and 48 hr), while exposure to 50 mg/kg lead acetate increased catalase activity (after 48 hr) and inhibited δ-aminolevulinate dehydratase activity (after 6, 24 and 48 hr) in the kidney (P < 0.05). Chronic exposure (30 days) to 5 mg/kg lead acetate inhibited δ-aminolevulinate dehydratase and increased glutathione S-transferase, non-protein thiol groups, catalase, thioredoxin reductase-1 and uric acid plasma levels, while exposure to 25 mg/kg lead acetate reduced body weight and δ-aminolevulinate dehydratase, but increased glutathione S-transferase, non-protein thiol groups and uric acid plasma levels (P < 0.05). No changes were observed in thiobarbituric acid reactive substances, glutathione peroxidase, creatinine or inorganic phosphate levels after either acute or chronic exposure. Our results suggest that thioredoxin reductase-1 may be an early indicator of acute exposure to low lead doses.

Document Type: Research article

DOI: 10.1111/j.1742-7843.2007.00084.x

Affiliations: 1: Postgraduate Program on Biochemical Toxicology, Center of Natural and Exact Sciences, Federal University of Santa Maria, and 2: Integrated Center for Laboratory Analysis Development (NIDAL), Department of Alimentary Technology and Science, Center of Rural Sciences, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

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Effect of Lead Acetate on Cytosolic Thioredoxin Reductase Activity and Oxidative Stress Parameters in Rat Kidneys