IngentaConnect Up-regulation of cyclooxygenase-2 during acute human renal allogr...

Authors: Rangel, Érika B¹; Moura, Luiz A²; Franco, Marcello F²; Pacheco-Silva, Álvaro¹

Source: Clinical Transplantation, Volume 19, Number 4, August 2005 , pp. 543-550(8)

Abstract:

Rangel ÉB, Moura LA, Franco MF, Pacheco-Silva Á. Up-regulation of cyclooxygenase-2 during acute human renal allograft rejection.

Clin Transplant 2005: 19: 543–550. © Blackwell Munksgaard, 2005 Abstract: Background:

Cyclooxygenases-1 and -2 (COX-1 and COX-2) are important in renal physiology and in many abnormal states. However, there is poor information about them during renal allograft rejection. The purpose of this study was to analyze cyclooxygenases expression in renal tissue allograft during acute rejection. Methods:

COX-1 and COX-2 transcripts and proteins were analyzed by semi-quantitative RT-PCR and immunohistochemistry in samples from human renal allografts obtained from nephrectomy because of irreversible acute rejection. Results:

In samples with acute rejection, we detected higher expression of COX-2 mRNA in comparison with COX-1 (p < 0.001) being COX-2 expression not different from COX-1 in samples from renal allografts without acute rejection. COX-1 and COX-2 localization was in accordance with data described in literature, however COX-2 protein was higher in interstitial cells in the group with rejection than in the group without rejection (p = 0.04). In addition, in samples with acute rejection COX-2 immunoreactivity was more prominent in podocytes (p < 0.001), in proximal tubules (p < 0.001), in collecting duct cells (p = 0.003) and in interstitial cells (p < 0.001) when compared with COX-1. Conclusions:

Our data show that there is an increased production of COX-2 during acute renal rejection.

Keywords: acute rejection; cyclooxygenases-1 and-2; renal allografts

Document Type: Research article

DOI: 10.1111/j.1399-0012.2005.00386.x

Affiliations: 1: Division of Nephrology 2: Department of Pathology, Hospital do Rim e Hipertensão and Universidade Federal de São Paulo, Brazil

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