IngentaConnect Tgf-1, Tgf-2, Tgf-3 and Msx2 expression is elevated during fronto...

Abstract:

Structured Abstract

Authors -Adab K, Sayne JR, Carlson DS, Opperman LA.

Objectives -It is hypothesized that regulation of facial suture morphogenesis is similar to that of cranial sutures, with expression of similar regulatory molecules, governing suture formation and patency. The present study was designed to characterize the morphology of the frontonasal (FN) suture of the rat at different developmental stages and to investigate the presence and temporal-spatial expression of transforming growth factor-beta 1 (Tgf-β1), Tgf-β2, Tgf-β3 and Msx2 mRNA within these structures.

Setting and sample population -The Department of Biomedical Sciences at Texas A&M University System Health Science Center, Baylor College of Dentistry, Dallas, TX USA. Histological sections and RNA isolated from FN suture tissues of Sprague-Dawley rats, aged embryonic day 16 through postnatal day 20.

Method -Sections were examined after immunohistochemical staining. Gene expression was determined by densitometric analysis of RT-PCR products run on agarose gels.

Results -FN sutures develop slightly later than cranial sutures and show increased complexity over time when compared to cranial sutures. FN sutures were closely associated with the nasal capsular cartilage, with intervening layers of perichondrium and periosteum. The pattern of expression of Tgf-βs within the FN suture tissues was similar to that seen in the cranial sutures. However, mRNA and protein of the Tgf-βs were differentially expressed over time compared to cranial sutures. In FN sutures, Tgf-β mRNA levels were elevated both during the period of suture morphogenesis and during active bone growth from the suture in the early postnatal period. Msx2 mRNA expression was elevated in both the prenatal and postnatal periods, similar to Tgf-β mRNA expression.

Conclusion -Tgf-β and Msx2 are present in facial sutures similar to cranial sutures, but are differenentially expressed over time, perhaps reflecting different bone growth rates from these sutures.

Keywords: craniofacial sutures; facial growth; morphogenesis; Msx2; transforming growth factor-βs

Document Type: Research article

DOI: 10.1034/j.1600-0544.2002.02227.x

Affiliations: 1: K. Adab, J.R. Sayne, D.S. Carlson, L.A. Opperman, Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA

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Tgf-β1, Tgf-β2, Tgf-β3 and Msx2 expression is elevated during frontonasal suture morphogenesis and during active postnatal facial growth