IngentaConnect Ceramic hydroxyapatite coating on titanium implants drives select...

Abstract:

The aim of this study was to determine the cell characteristics that regulate implant osseointegration. The heterogeneity of bone marrow stromal cells obtained from 11 donors was assessed by measuring the expression of a large panel of adhesion molecules. Large differences in expression of adhesion molecules were detected depending on the culture conditions used. Cells cultured in fetal bovine serum induced the expression of different adhesion molecules from cells cultured in human serum. Donor-to-donor variation was determined by measuring the expression of adhesion molecules for stromal cells obtained from different donors that were processed identically. Fat adherent cells but also loose bone marrow cells showed large differences in expression of some but not all adhesion molecules. The flow cytometric data demonstrated large heterogeneity in expression of adhesion molecules, and this heterogeneity was influenced by culture conditions and varied from donor to donor. This demonstrates that the implant encounters different cell types, which could lead to different levels of integration. Surprisingly, in vitro only a subfraction of bone marrow stromal cells attached to titanium coated with ceramic hydroxyapatite. Adaptation of all cell types present in heterogeneous bone marrow to a coated surface is apparently not possible. Differential binding was not caused by aberrant staining of the stromal cells as the results were confirmed with bone marrow cells obtained from transgenic GFP mice. These results demonstrate that hydroxyapatite ceramics are selective in cell recruitment from the bone marrow, explaining the differences found in vivo for these coatings compared with titanium.

To cite this article:

Torensma R, ter Brugge PJ, Jansen JA, Figdor CG. Ceramic hydroxyapatite coating on titanium implants drives selective bone marrow cell adhesion.

Clin. Oral Impl. Res.14, 2003; 569–577.

Keywords: bone marrow stromal cells; ceramic coating; titanium implants

Document Type: Research article

DOI: 10.1034/j.1600-0501.2003.00949.x

Affiliations: 1: Department of Tumor Immunology, UMC Nijmegen, Nijmegen, the Netherlands. 2: Department of Biomaterials, UMC Nijmegen, Nijmegen, the Netherlands

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Ceramic hydroxyapatite coating on titanium implants drives selective bone marrow stromal cell adhesion