IngentaConnect Autoantibodies in Argentine Women with Recurrent Pregnancy Loss

Authors: Bustos, Daniel¹; Moret, Ana¹; Tambutti, Monica²; Gogorza, Sebastian³; Testa, Roberto³; Ascione, Amanda¹; Prigoshin, Norma²

Source: American Journal of Reproductive Immunology, Volume 55, Number 3, March 2006 , pp. 201-207(7)

Publisher: Blackwell Publishing

Abstract:

Problem

To determine the presence or absence of subclinical autoimmunity in Caucasian Argentine healthy women with first trimester recurrent pregnancy loss (RPL), the sera of 118 healthy women with a history of three or more consecutive abortions and 125 fertile control women without abortions and two children were analyzed for the presence of autoantibodies: immunoglobulin (Ig)G and IgM anticardiolipin, antinuclear (ANA), antismooth muscle (ASMA), antimitocondrial (AMA), antiliver-kidney-microsomal fraction (LKM), antigastric parietal cells (GPC), antineutrophil cytoplasmatic (ANCA) and antibodies antigliadin type IgA and IgG and IgA antitransglutaminase related with celiac disease (CD). Method of study

ANA, ASMA, AMA, anti-LKM, antibodies to GPC and ANCA were determined by indirect immunofluorescence (IFI) and anticardiolipin, antigliadina and antitransglutaminase antibodies were measured by enzyme-linked immunosorbent assays (ELISA). Results

There was no significant difference between controls and patients with ANA, ASMA, AMA, LKM, ANCA and GPC. The prevalence of anticardiolipin antibodies in RPL was significantly higher than controls (P < 0,01) and the prevalence of positive antibodies for antigliadina type IgA and IgG and IgA antitransglutaminase in RPL was significantly higher than controls (P < 0.04). Conclusion

We show that Caucasian Argentine women with RPL showed significantly higher incidence of anticardiolipin antibodies than normal controls and finally we recommended the screening of IgA and IgG antigliadina and IgA antitransglutaminase antibodies in pregnancy, because of the high prevalence of subclinical CD in RPL and the chance of reversibility through consumption of a gluten free diet.

Keywords: Autoantibodies; autoimmunity; recurrent pregnancy loss

Document Type: Research article

DOI: 10.1111/j.1600-0897.2005.00349.x

Affiliations: 1: Central Laboratory, Diagnostic Department, Hospital Italiano de Buenos Aires, Gascón 450, Buenos Aires, Argentina; 2: Histocompatibility and Immunogenetics laboratory, Diagnostic Department, Hospital Italiano de Buenos Aires, Gascón 450, Buenos Aires, Argentina; 3: Reproduction Section, Gynecology Department, Hospital Italiano de Buenos Aires, Gascón 450, Buenos Aires, Argentina

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