@article {Hai:February 2008:0161-6412:99,
	author = "Hai, Jian",
	author = "Lin, Qi",
	author = "Lu, Yang",
	author = "Yi, Jing",
	author = "Zhang, Hao",
	title = "Growth inhibition and induction of differentiation by panaxydol in rat C6 glioma cells",
	journal = "Neurological Research",
	volume = "30",
	year = "February 2008",
	abstract = "<B>Objectives:</B> Panaxydol is a naturally occurring non-peptidyl small molecule isolated from the lipophilic fractions of Panax notoginseng, a well-known Chinese traditional medicine. In this study, we aimed to investigate the effects of panaxydol on growth inhibition and its mechanisms in C6 rat glioma cells.<P></P><B>Methods:</B> The effects of panaxydol on cell proliferation, morphologic changes, glial fibrillary acidic protein (GFAP) expression and cell cycle regulation in rat C6 cells were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, hematoxylin and eosin (HE) staining, immunocytochemistry, flow cytometric analysis and Western blot respectively.<P></P><B>Results:</B> Panaxydol markedly inhibited the proliferation of C6 cells in a dose-dependent manner with IC<SUB>50</SUB> of 39.5 &#177; 2.3 &#956;M. In addition, the cell morphologic changes and increased expression of GFAP in C6 cells in the presence of panaxydol implied a cellular differentiation. Flow cytometric analysis revealed that panaxydol-treated cells accumulated in G<SUB>0</SUB>/G<SUB>1</SUB> phase with a marked decrease in the number of C6 cells at S phase. Western blot analysis demonstrated that panaxydol resulted in an increase in the protein expression of p27 in C6 cells as early as 3 hours after treatment consistent with the differentiation response, but protein expression of p53, p21, p16 and pRb remained unchanged.<P></P><B>Conclusion:</B> These findings suggest that panaxydol inhibits the proliferation of C6 cells via G<SUB>0</SUB>/G<SUB>1</SUB> cell cycle arrest in association with induction of p27 expression and differentiation.",
	pages = "99-105(7)",
	url = "http://www.ingentaconnect.com/content/maney/nres/2008/00000030/00000001/art00014"
	doi = "doi:10.1179/016164107X228697"
}