@article {Komotar:September 2005:0161-6412:634, author = "Komotar, Ricardo J.", author = "Mocco, J.", author = "Mack, William J.", author = "Ransom, Evan R.", author = "Zacharia, Brad E.", author = "King, Ryan G.", author = "Ducruet, Andrew F.", author = "Cohen, Hilary G.", author = "Arango, Victoria", author = "Connolly, E. Sander", title = "Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke", journal = "Neurological Research", volume = "27", year = "September 2005", abstract = "Objectives: Infarct volume correlation using magnetic resonance imaging (MRI) and pathology specimens enables exact tissue localization of cerebral injury following experimental stroke. We describe a protocol that enables co-registration of radiographic signal change and histologic ischemia in a non-human primate model of stroke.

Methods: One male baboon underwent left middle cerebral artery territory occlusion/reperfusion. MRI [5 mm axial T2 weighted (T2W) slices] was carried out 9 days post-ischemia after which the animal was killed. Immediately post-mortem, the whole brain was perfused and fixed in paraformaldehyde and sliced into 5 mm axial sections that corresponded to those demonstrated on MRI. Slices (40 mum) were obtained from each section and were then stained using Luxol hematoxylin and eosin.

Results: The relative area of hyperintensity demonstrated on T2W MRI approximates, in size and location, the region of infarct on gross pathology. This was confirmed microscopically.

Discussion: With the use of advanced imaging modalities, this co-registration technique affords the capacity to differentiate ischemic core, penumbra, and uninjured cortex following experimental stroke. Such a precise delineation enables immunohistochemical analysis of a wide variety of substrates in each of the aforementioned regions.", pages = "634-637(4)", url = "http://www.ingentaconnect.com/content/maney/nres/2005/00000027/00000006/art00011" doi = "doi:10.1179/016164105X17305" }