@article {Rivera:December 2008:0003-4983:671,
	author = "Rivera, W.L.",
	author = "Adao, D.E.V.",
	title = "Identification of the 18S-ribosomal-DNA genotypes of Acanthamoeba isolates from the Philippines",
	journal = "Annals of Tropical Medicine and Parasitology",
	volume = "102",
	year = "December 2008",
	abstract = "Cyst morphology has been commonly used to identify the free-living amoeba <I>Acanthamoeba</I> to subgenus level. A more accurate and consistent method, based on the sequence analysis of the gene coding for the amoeba's small-subunit ribosomal RNA (<I>Rns</I>), has, however, been developed. There have been no attempts to identify the <I>Acanthamoeba</I> genotypes circulating in the Philippines. In this study, therefore, the ASA.S1 region of the <I>Rns</I> gene from 17 <I>Acanthamoeba</I> isolates, collected from soil, water and contact-lens storage cases in different regions of the Philippines, was sequenced. After the isolates were genotyped, using the BLAST program, their phylogenetic positions relative to known <I>Acanthamoeba</I> isolates were determined. For this, the model-based (GTR + &#915;) neighbour-joining, maximum-likelihood and Bayesian-inference analyses and the non-model-based maximum-parsimony analysis were used. All but two of the isolates were identified as the T5 or T4 genotypes, which are probably common in soil, water and contact-lens cases across the Philippines. The only other genotypes identified were T15 (as a single isolate from a contact-lens case) and T3 (as a single soil isolate).",
	pages = "671-677(7)",
	url = "http://www.ingentaconnect.com/content/maney/atmp/2008/00000102/00000008/art00002"
	doi = "doi:10.1179/136485908X337544"
}