@article {Zavvar:October 2008:0003-4983:597,
	author = "Zavvar, M.",
	author = "Sadraei, J.",
	author = "Emadi, H.",
	author = "Pirestani, M.",
	title = "The use of a nested PCRRFLP technique, based on the parasite's 18S ribosomal RNA, to characterise Cryptosporidium isolates from HIV/AIDS patients",
	journal = "Annals of Tropical Medicine and Parasitology",
	volume = "102",
	year = "October 2008",
	abstract = "Since there have been few studies on human cryptosporidiosis in Iran, attempts were made to identify <I>Cryptosporidium</I> isolates from HIV-positive Iranians, to genotype level. A nested PCR (based on a fragment of the parasite's 18S ribosomal-RNA gene) was first used to see if faecal samples from 35 HIV-positive patients (of whom 17 had apparently been found smear-positive for <I>Cryptosporidium</I> oocysts) contained <I>Cryptosporidium</I>. Twenty-one of the samples (including all 17 of those that appeared smear-positive) were found PCR-positive. Each of these 21 samples was then investigated further, by RFLP analysis in which the amplicons from the secondary PCR were digested with <I>Vsp</I>I. Curiously, although HIV-infected individuals are known to be susceptible to infection with a wide range of <I>Cryptosporidium</I> genotypes, all the Iranian subjects of the present study were found to be infected with <I>C. hominis</I> (71%) or <I>C. parvum</I> (29%).",
	pages = "597-601(5)",
	url = "http://www.ingentaconnect.com/content/maney/atmp/2008/00000102/00000007/art00004"
	doi = "doi:10.1179/136485908X311876"
}