@article {Bachand:1 June 2000:1387-2176:179,
	author = "Bachand, G.D.",
	author = "Montemagno, C.D.",
	title = "Constructing Organic/Inorganic NEMS Devices Powered by Biomolecular Motors",
	journal = "Biomedical Microdevices",
	volume = "2",
	year = "1 June 2000",
	abstract = "The recognition of many enzymes as nanoscale molecular motors has opened the door for the potential creation of hybrid organic/inorganic nano-electro-mechanical (NEMS) devices. The long-term objective of this research is the integration of F_1-ATPase with NEMS to produce useful nanoscale devices. A thermostable F_1-ATPase coding sequence has been isolated, cloned, and engineered for high-level protein expression. Precise positioning, orientation, and spacing of individual F_1-ATPase molecules were achieved on patterned nickel arrays produced using electron beam lithography. An efficient and accurate assay was developed to evaluate the performance of individual F_1-ATPase motors, and confirmed a three-step mechanism of γ subunit rotation during ATP hydrolysis. Further assessment of the biophysical and bioengineering properties of F_1-ATPase currently are being conducted, as well as the construction of a hybrid NEMS device powered by F_1-ATPase. The evolution of this technology will permit the creation of novel classes of nanoscale, hybrid devices.",
	pages = "179-184(6)",
	url = "http://www.ingentaconnect.com/content/klu/bmmd/2000/00000002/00000003/00264805"
}