@article {Joong:February 1999:0951-208X:145,
	author = "Joong Kwon Y.",
	author = "Hatti-Kaul R.",
	title = "Protein separation using metal ion-bound particles in aqueous two-phase system",
	journal = "Biotechnology Techniques",
	volume = "13",
	year = "February 1999",
	abstract = "<P>Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.</P>",
	pages = "145-148(4)",
	url = "http://www.ingentaconnect.com/content/klu/bite/1999/00000013/00000002/00205943"
}