@article {Deak:March 2003:0141-5492:397,
	author = "Deak P.M.",
	author = "Lutz-Wahl S.",
	author = "Bothe H.",
	author = "Fischer L.",
	title = "Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis",
	journal = "Biotechnology Letters",
	volume = "25",
	year = "March 2003",
	abstract = "<P>The penicillin G amidase (PGA) from <I>Alcaligenes faecalis</I>, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in <I>Escherichia coli</I>. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5&#160;l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.</P>",
	pages = "397-400(4)",
	url = "http://www.ingentaconnect.com/content/klu/bile/2003/00000025/00000005/05118815"
}