@article {Cheng:December 2008:0362-028X:2436,
	author = "Cheng, Chorng-Ming",
	author = "Lin, Wen",
	author = "Thien Van, Khanh",
	author = "Phan, Lieuchi",
	author = "Tran, Nelly N.",
	author = "Farmer, Doris",
	title = "Rapid Detection of Salmonella in Foods Using Real-Time PCR",
	journal = "Journal of Food Protection®",
	volume = "71",
	year = "December 2008",
	abstract = "Conventional methods for detection of <I>Salmonella</I> serovars in foods are generally time-consuming and labor intensive. A real-time PCR method has been developed with custom designed primers and a TaqMan probe to detect the presence of a 262-bp fragment of the <I>Salmonella</I>-specific <I>invA</I> gene. The method has been tested with a total of 384 field-isolated <I>Salmonella</I> serovars and non-<I>Salmonella</I> stock strains, as well as 420 U.S. Food and Drug Administration food samples, comprising a variety of food matrices. The method was highly specific in detecting <I>Salmonella</I> in spiked chili powder and shrimp samples, with a sensitivity of 0.04 CFU/g. In addition, the method is faster, more accurate, and less costly than the traditional U.S. Food and Drug Administration's <I>Bacteriological Analytical Manual</I> cell-culturing and the AOAC International-approved VIDAS methods to detect <I>Salmonella</I> in foods.",
	pages = "2436-2441(6)",
	url = "http://www.ingentaconnect.com/content/iafp/jfp/2008/00000071/00000012/art00009"
}