@article {Shin:September 2004:1542-6416:235,
	author = "Shin, Yong-seung",
	author = "Lee, Eung-goo",
	author = "Shin, Gee-wook",
	author = "Kim, Young-rim",
	author = "Lee, Eun-young",
	author = "Kim, Jae-hoon",
	author = "Jang, Hwan",
	author = "Kim, Dae-yong",
	author = "Kim, Yong-hwan",
	author = "Kim, Gon-sup",
	author = "Suh, Myung-deuk",
	author = "Jung, Tae-sung",
	title = "Development of competitive ELISA for neosporosis by employing immunoproteomics",
	journal = "Clinical Proteomics",
	volume = "1",
	year = "September 2004",
	abstract = "In this study, proteomics was used to explore the antigenic proteins that are involved in cross-reactivity during serodiagnosis between <I>Neospora caninum (N. caninum)</I> and <I>Toxoplasma gondii (T. gondii)</I>. Competitive enzyme-linked immunosorbent assay (C-ELISA) developed by proteomics shed a new light on the infection of <I>N. caninum</I>. Cross-reactivity of antigenic proteins between <I>N. caninum</I> and <I>T. gondii</I> tachyzoites was explored by using the conventional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) (1-DE) and two-dimensional gel electrophoresis (2-DE) immunoblot. The proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The protein expression patterns in the immunoblot profiles of <I>N. caninum</I> were similar to bovine, chicken, and rabbit anti-<I>N. caninum</I> serum, but they were not similar to rabbit anti-<I>T. gondii</I> serum. Band at 79 kDa, HSP70, and actin on immunoblot profiles reacted, in general, with bovine, chicken, and rabbit anti-<I>N. caninum</I> serum and also with rabbit anti-<I>T. gondii</I> serum, respectively. Whereas the band at 144 kDa, and NCDG-1 were detected on bovine, chicken, and rabbit anti-<I>N. caninum</I> immunoblot profiles, they were not observed on rabbit anti-<I>T. gondii</I> immunoblot profile. These specific antigenic proteins were recorded as species-specific proteins of <I>N. caninum</I> against <I>T. gondii</I>. Based on the proteome analysis, C-ELISA was developed to screen the cattle infected with <I>N. caninum</I> by using <I>N. caninum</I> tachyzoite lysate as a coating antigen and chicken anti-<I>N. caninum</I> immunoglobulin (Ig)Y as a competitor. C-ELISA was able to detect the antibody of <I>N. caninum</I> without cross-reactivity with <I>T. gondii</I>. Furthermore, it achieved a fine diagnostic performance in the cases of 162 bovine sera.",
	pages = "235-248(14)",
	url = "http://www.ingentaconnect.com/content/hum/cp/2004/00000001/F0020003/art00002"
	doi = "doi:10.1385/CP:1:3-4:235"
}