Enhancement of Viability of Fat Grafts in Nude Mice by Endothelial Progenitor Cells

Authors: YI, CHENGGANG1; PAN, YONG1; ZHEN, YAN1; ZHANG, LINXI1; ZHANG, XUDONG1; SHU, MAOGUO1; HAN, YAN1; GUO, SHUZHONG1

Source: Dermatologic Surgery, Volume 32, Number 12, December 2006 , pp. 1437-1443(7)

Publisher: Blackwell Publishing

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Abstract:

BACKGROUND

A recent discovery showed that endothelial progenitor cells (EPCs) could augment collateral vessel growth to ischemic tissues. OBJECTIVE

The objective was to demonstrate the effects of EPCs on the vasculogenesis and survival of free transplanted fat tissues in nude mice. METHODS

EPCs from human donors were cultured in vitro for 7 days. Human fat tissues were injected subcutaneously into the scalps of 20 6-week-old nude male mice. EPCs stained with CM-DiI were mixed with the transplanted fat tissues and injected into the mice. EBM-2 medium was used as control group. The animals were euthanized 15 weeks after the procedure. Graft volume were measured, and histologic evaluation was performed. The central part of fat tissues was histologically evaluated 15 weeks after the fat injection. RESULTS

The survival volume of the experimental group was significantly greater than that of the control group (p< .05). Less cyst formation and fibrosis was obtained in the experimental group. Histologic evaluation of the central part of fat tissues 15 weeks after the fat injection showed that capillary densities increased markedly in the experimental group mice. CONCLUSION

The results indicate that EPCs have the ability to enhance the survival and the quality of the transplanted fat tissues.

This study was supported by a grant of the Natural Nature Science Foundation of China (No. 30400471).

Document Type: Research article

DOI: 10.1111/j.1524-4725.2006.32351.x

Affiliations: 1: All authors are affiliated with the Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, China

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