IngentaConnect Bartonella henselae inhibits apoptosis in Mono Mac 6 cells

Authors: Volkhard A. J. Kempf¹; Annette Schairer¹; Diana Neumann¹; Guntram A. Grassl¹; Kirsten Lauber²; Maria Lebiedziejewski¹; Martin Schaller³; Pierre Kyme¹; Sebastian Wesselborg²; Ingo B. Autenrieth¹

Source: Cellular Microbiology, Volume 7, Number 1, January 2005 , pp. 91-104(14)

Publisher: Blackwell Publishing

Abstract:

Summary

Bartonella henselae causes the vasculoproliferative disorders bacillary angiomatosis and peliosis probably resulting from the release of vasculoendothelial growth factor (VEGF) from infected epithelial or monocytic host cells. Here we demonstrate that B. henselae in addition to VEGF induction was also capable of inhibiting the endogenous sucide programme of monocytic host cells. Our results show that B. henselae inhibits pyrrolidine dithiocarbamate (PDTC)-induced apoptosis in Mono Mac 6 cells. B. henselae was observed to be present in a vacuolic compartment of Mono Mac 6 cells. Direct contact of B. henselae with Mono Mac 6 cells was crucial for inhibition of apoptosis as shown by the use of a two-chamber model. Inhibition of apoptosis was paralleled by diminished caspase-3 activity which was significantly reduced in PDTC-stimulated and B. henselae-infected cells. The anti-apoptotic effect of B. henselae was accompanied by (i) the activation of the transcription factor NF- kgr

B and (ii) the induction of cellular inhibitor of apoptosis proteins-1 and -2 (cIAP-1, -2). Our results suggest a new synergistic mechanism in B. henselae pathogenicity by (i) inhibition of host cell apoptosis via activation of NF- kgr

B and (ii) induction of host cell VEGF secretion.

Document Type: Research article

DOI: 10.1111/j.1462-5822.2004.00440.x

Affiliations: 1: Institut für Medizinische Mikrobiologie und Hygiene, 2: Abteilung Innere Medizin and 3: Universitäts-Hautklinik, Abteilung Innere Medizin I, Eberhard-Karls-Universität, Tübingen, Germany.

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