BLOCKADE OF β1- AND β2-ADRENOCEPTORS DELAYS WOUND CONTRACTION AND RE-EPITHELIALIZATION IN RATS

Authors: Souza, Bruna R; Santos, Jeanine S; Costa, Andréa MA

Source: Clinical and Experimental Pharmacology and Physiology, Volume 33, Numbers 5-6, May/June 2006 , pp. 421-430(10)

Publisher: Blackwell Publishing

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Abstract:

SUMMARY

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The participation of sympathetic efferent fibres in wound healing is not well understood. The aim of the present study was to investigate the effects of b1- and b2-adrenoceptor blockade on rat excisional cutaneous wound healing.

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Male rats were treated orally with propranolol dissolved in drinking water (50 mg/kg per day), whereas the control group received drinking water without propranolol. Propranolol was administered daily until rats were killed. A full-thickness excisional lesion was performed. The lesion area was measured to evaluate wound contraction. After rats had been killed, lesion and adjacent normal skin were formol fixed and paraffin embedded. Sections were stained with haematoxylin–eosin, Sirius red or Toluidine blue and immunostained for a-smooth muscle actin or proliferating cell nuclear antigen.

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Propranolol-treated rats presented delayed wound contraction and epidermal healing and decreased hydroxyproline levels, collagen density and neo-epidermis thickness. Blockade of b1- and b2-adrenoceptors increased epidermal and connective tissue cell proliferation, polymorphonuclear leucocyte migration, myofibroblast density and mast cell migration. The volume density of blood vessels was increased and vessels were more dilated in propranolol-treated animals.

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Thus, we conclude that b1- and b2-adrenoceptor blockade impairs cutaneous wound healing. This information should be considered by physicians during the treatment of patients who present with hypertension and problems in the healing process (such as venous ulcers).

Keywords: β-adrenoceptors; fibroblasts; mast cells; propranolol; wound healing

Document Type: Research article

DOI: 10.1111/j.1440-1681.2006.04383.x

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