IngentaConnect USE OF GLUT-4 NULL MICE TO STUDY SKELETAL MUSCLE GLUCOSE UPTAKE

Authors: Charron, Maureen J¹; Gorovits, Naira²; Laidlaw, J Skye²; Ranalletta, Mollie²; Katz, Ellen B²

Source: Clinical and Experimental Pharmacology and Physiology, Volume 32, Number 4, April 2005 , pp. 308-313(6)

Abstract:

SUMMARY

1. The present review focuses on the effects of varying levels of GLUT-4, the insulin-senstive glucose transporter, on insulin sensitivity and whole body glucose homeostasis.

2. Three mouse models are discussed including myosin light chain (MLC)-GLUT-4 mice which overexpress GLUT-4 specifically in skeletal muscle, GLUT-4 null mice which express no GLUT-4 and the MLC-GLUT-4 null mice which express GLUT-4 only in skeletal muscle. Overexpressing GLUT-4 specifically in the skleletal muscle results in increased insulin sensitivity in the MLC-GLUT-4 mice. In contrast, the GLUT-4 null mice exhibit insulin intolerance accompanied by abnormalities in glucose and lipid metabolism. Restoring GLUT-4 expression in skeletal muscle in the MLC-GLUT-4 null mice results in normal glucose metabolism but continued abnormal lipid metabolism.

3. The results of experiments using these mouse models demonstrates that modifying the expresssion of GLUT-4 profoundly affects whole body insulin action and consequently glucose and lipid metabolism.

Keywords: glucose transporter; glucose uptake; GLUT-4; insulin action; skeletal muscle

Document Type: Research article

DOI: 10.1111/j.1440-1681.2005.04189.x

Affiliations: 1: Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, New York, USA 2: Biochemistry and

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