IngentaConnect Effects of caffeic acid phenethyl ester on glycerol-induced acute...

Authors: Nurettin Aydogdu¹; Gulizar Atmaca¹; Omer Yalcin²; Kadir Batcioglu³; Kadir Kaymak¹

Source: Clinical and Experimental Pharmacology and Physiology, Volume 31, Number 9, September 2004 , pp. 575-579(5)

Abstract:

Summary

1. Free radicals and nitric oxide (NO) play a crucial role in the pathogenesis of myoglobinuric acute renal failure (ARF). The aim of the present study was to investigate the effects of caffeic acid phenethyl ester (CAPE), an anti-oxidant, on the myoglobinuric ARF induced by intramusculer hypertonic glycerol injection.

2. Thirty rats were divided equally into three groups. Rats in group 1 were given saline and those in groups 2 and 3 were injected with glycerol (10 mL/kg, i.m.). Concomitant and 24 h after glycerol injection, CAPE (10 µmol/kg, i.p.) was administered to group 3 rats. Forty-eight hours after glycerol injection, blood samples and kidney tissues of rats were taken under anaesthesia.

3. Plasma concentrations of urea, creatinine, malondialdehyde (MDA) and NO were determined, as were superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities and MDA levels in kidney tissues. Kidney morphology was also investigated.

4. In the group receiving CAPE, although SOD enzyme activity was found to be increased, we failed to find any protective effect of CAPE on other parameters investigated. Moreover, although CAPE significantly decreased NO levels, it increased plasma concentrations of urea and MDA.

5. We suggest that the effect of CAPE in decreasing NO concentrations may further increase the renal ischaemia in this model. Thus, CAPE may have a worsening rather than beneficial effect under these conditions in this model of ARF.

Keywords: acute renal failure; anti-oxidant enzymes; caffeic acid phenethyl ester; glycerol; lipid peroxidation; nitric oxide

Document Type: Research article

DOI: 10.1111/j.1440-1681.2004.04050.x

Affiliations: 1: Physiology and 2: Pathology, Faculty of Medicine, University of Trakya, Edirne and 3: Department of Biochemistry, Faculty of Pharmacy, University of Inonu, Malatya, Turkey

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

Effects of caffeic acid phenethyl ester on glycerol-induced acute renal failure in rats