IngentaConnect Chronic Hypoxia Preconditioning Increases Survival In Rats Suffer...

Authors: Wen, Hsiao-Chuan¹; Lee, Chin-Cheng²; Lee, Wen-Chuan³; Huang, Kuo-Sheng⁴; Lin, Mao-Tsun¹

Source: Clinical and Experimental Pharmacology and Physiology, Volume 29, Numbers 5-6, May/June 2002 , pp. 435-440(6)

Publisher: Blackwell Publishing

Abstract:

SUMMARY

1. In the present study, we assessed the protective effects of chronic hypoxia preconditioning against heatstroke-induced injury in urethane-anaesthetized rats. Heatstroke was induced by exposing the animals to an ambient temperature of 42°C. The time at which both the mean arterial pressure (MAP) and local cerebral blood flow (CBF) in the striatum began to decrease from peak levels was taken as the onset of heatstroke. Control rats were exposed to a temperature of 24°C.

2. Mean arterial pressure, CBF, blood pH, P_aO₂, P_aCO₂ and survival time (the interval between onset of heatstroke and cardiac arrest) after heat stress were all lower than in control rats (in which `survival time' was defined as > 360 min). However, blood lactate concentrations were greater in rats exposed to heat. Rats placed at high altitude (HA), when exposed to the same heat stress (42°C) survived much longer (113 ± 26 min; n = 8) than rats maintained at sea level (SL; 20 ± 2 min; n = 8).

3. After the onset of heatstroke, blood pH and lactate concentrations were found to be significantly higher and lower, respectively, in HA rats than in SL rats.

4. Western blot assay revealed that chronic hypoxia preconditioning induced heat shock protein (HSP) 72 expression in both the kidneys and lungs.

5. Thus, it appears that the observed benefit of chronic hypoxia preconditioning is related to attenuation of tissue acidification and elevations of HSP72 expression in both kidneys and lungs during heatstroke.

Keywords: chronic hypoxia; heat shock protein; heatstroke; high altitude; tissue acidification

Document Type: Research article

DOI: 10.1046/j.1440-1681.2002.03680.x

Affiliations: 1: Institute of Physiology, National Yang-Ming University, 2: Department of Pathology and Laboratory Medicine, Shin-Kong Hospital, Taipei, 3: Department of Comparative Medicine, Pig Research Institute, Chunan, Maioli and 4: Department of Internal Medicine, Hsin-Chu Hospital, Hsin-Chu,

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Chronic Hypoxia Preconditioning Increases Survival In Rats Suffering From Heatstroke