Polymorphisms Of The Renin Gene Promoter In Spontaneously Hypertensive And Wistar-Kyoto Rats

Author: Leckie, Brenda J

Source: Clinical and Experimental Pharmacology and Physiology, Volume 28, Numbers 1-2, January/February 2001 , pp. 60-63(4)

Publisher: Blackwell Publishing

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Abstract:

SUMMARY

1. In the present study, 1.39 kb of the renin gene 5′ region in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was amplified by polymerase chain reaction from genomic DNA and sequenced. Consistent differences in the renin gene sequence of SHR and WKY rats were found at positions -725, -727, -979 and -1126/-1129 as numbered from the transcription start site (+1). No polymorphism was specific to hypertensive rats.

2. Gel-shift assays were performed using labelled SHR renin promotor DNA and nuclear proteins extracted from rat kidneys. The regions between -1122 and -1139 and between -701 and -797 showed protein binding.

Keywords: renin gene polymorphism; renin gene promoter; spontaneously hypertensive rats

Document Type: Short communication

DOI: 10.1046/j.1440-1681.2001.03397.x

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