IngentaConnect Role Of Nitric Oxide Synthase Inhibitor In Experimental Colitis I...

Authors: Hosoi, Tsutomu¹; Goto, Hidemi¹; Arisawa, Tomiyasu¹; Niwa, Yasumasa¹; Okada, Naoaki¹; Ohmiya, Naoki¹; Hayakawa, Tetsuo¹

Source: Clinical and Experimental Pharmacology and Physiology, Volume 28, Numbers 1-2, January/February 2001 , pp. 9-12(4)

Publisher: Blackwell Publishing

Abstract:

SUMMARY

1. The present study was designed to investigate the role of nitric oxide (NO) in modulating 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats.

2. Damage scores and NO synthase (NOS) activity were measured.

3. The damage scores and NOS activity reached a peak on the 4th day after administration of TNBS solution (day 0), thereafter gradually decreasing, and were significantly higher than in the group treated with saline throughout the experimental period.

4. Subsequently, we divided the stage of colitis into two groups, one from day 0 to day 3 after induction of colitis, and the other from day 4 onwards. We evaluated the effects of the NOS inhibitor N^G-monomethyl-L-arginine (L-NMMA), on TNBS-hapten-induced colitis and colonic mucosal blood flow. Two different methods ofL-NMMA administration, from day 0 to day 3, and from day 4 onwards, were undertaken.

5. The damage score in the earlyL-NMMA treatment group was significantly higher than in the group withoutL-NMMA on day 14. In contrast, the damage score in the lateL-NMMA treatment group was not significantly different from the group withoutL-NMMA. Colonic mucosal blood flow in the earlyL-NMMA treatment group was not significantly different from that in the lateL-NMMA treatment group.

6. These data suggest that NO is important for inhibiting inflammation during the early stages.

Keywords: colonic mucosal blood flow; nitric oxide synthase; nitric oxide synthase inhibitor; 2; 4; 6-trinitrobenzene sulphonic acid colitis

Document Type: Research article

DOI: 10.1046/j.1440-1681.2001.03388.x

Affiliations: 1: Second Department of Internal Medicine and

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Role Of Nitric Oxide Synthase Inhibitor In Experimental Colitis Induced By 2,4,6-Trinitrobenzene Sulphonic Acid In Rats