IngentaConnect Localization of voltage-sensitive L-type calcium channels in the ...

Authors: Firth, Sally I; Morgan, Ian G¹; Boelen, Meeuwis K²; Morgans, Catherine W³

Source: Clinical & Experimental Ophthalmology, Volume 29, Number 3, June 2001 , pp. 183-187(5)

Abstract:

L-type calcium channels have been associated with synaptic transmission in the retina, and are a potential site for modulation of the release of neurotransmitters. The present study documents the immunohistochemical localization of neuronal α1 subunits of L-type calcium channels in chicken retina, using antibodies to the α1c, α1d and α1f subunits of L-type calcium channels. The α1c-like subunits were localized to Müller cells, with predominantly radial processes, and a prominent band of horizontal processes in the outer plexiform layer. The antibody to α1d subunits labelled most, if not all, cell bodies. The antibody to a human α1f subunit strongly labelled photoreceptor terminals. Fainter immunoreactivity was detected in the inner segments of the photoreceptors, a subset of amacrine cells, two bands of labelling in the inner plexiform layer and many ganglion cells. The differential cellular distributions of these α1-subunits suggests subtle functional differences in their roles at different cellular locations.

Keywords: α1 subunits; calcium channels; chicken; immunohistochemistry; Müller cells; retina

Document Type: Research article

DOI: 10.1046/j.1442-9071.2001.00401.x

Affiliations: 1: Visual Sciences Group, Research School of Biological Sciences & Centre for Visual Science, Australian National University, Canberra, Australian Capital Territory, 2: Division of Biological Sciences, La Trobe University, Bendigo, Victoria, and 3: Division of Neuroscience, John Curtin School for Medical Research, Australian National University, Canberra, Australian Capital Territory, Australia

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Localization of voltage-sensitive L-type calcium channels in the chicken retina