IngentaConnect Regulatory role of IL-1 in the expression of IL-6 and IL-8 in hum...

Authors: Xue, Mei-Lang¹; Willcox, Mark DP¹; Lloyd, Andrew²; Wakefield, Denis²; Thakur, Archana¹

Source: Clinical & Experimental Ophthalmology, Volume 29, Number 3, June 2001 , pp. 171-174(4)

Abstract:

Pseudomonas aeruginosa is a virulent pathogen and is frequently associated with bacterial keratitis. Recent studies have shown that high levels of interleukin (IL)-1β and macrophage inflammatory protein-2 are associated with the severity of corneal infection. Interleukin-1β is a principal inflammatory mediator. Understanding the regulatory role of IL-1β would provide better understanding of host responses during P. aeruginosa corneal infection. A human corneal epithelial (HCE) cell line and three P. aeruginosa strains were used in this experiment. Confluent HCE cells were challenged with P. aeruginosa and monoclonal antihuman IL-1β antibody (IL-1β mAb). The culture supernatants were collected for measuring cytotoxicity and protein levels of IL-1β, IL-8 and IL-6 by enzyme-linked immunosorbent assay. Results showed that HCE cells expressed low levels of IL-1β and high levels of IL-6 and IL-8 during P. aeruginosa colonization. Paer1-colonized HCE cells produced higher levels of IL-1β, IL-6 and IL-8 protein compared to those produced by 6206- and 6294- colonized HCE cells. Administration of IL-1β mAb decreased the production of IL-8 and IL-6. In conclusion, P. aeruginosa-colonized HCE cells produced low levels of IL-1β and high levels of IL-6 and IL-8. Neutralizing IL-1β protein significantly downregulated the production of IL-8 and IL-6.

Keywords: cytokines; human corneal epithelial cell; IL-1β; Pseudomonas aeruginosa

Document Type: Research article

DOI: 10.1046/j.1442-9071.2001.00410.x

Affiliations: 1: Cooperative Research Centre For Eye Research and Technology, and 2: School of Pathology, Inflammation Research Unit, The University of New South Wales, Sydney, New South Wales, Australia

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

Regulatory role of IL-1β in the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colonization