Random amplified polymorphic DNA analysis of Acinetobacter species isolated from worn contact lenses

Authors: Corrigan, Karen M; Zhu, Hua; Willcox, Mark DP

Source: Clinical & Experimental Ophthalmology, Volume 29, Number 3, June 2001 , pp. 153-156(4)

Publisher: Blackwell Publishing

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Abstract:

The purpose of this study was to explore the genotypes of Acinetobacter species and to compare the genotypes and phenotypes of the Acinetobacter isolated from contact lens wearers. Nineteen strains were used in the study, 13 were isolated from patients experiencing an adverse response event, and six strains were isolated from asymptomatic contact lens wearers. Random amplified polymorphic DNA and phenotypic analyses were carried out using commercially available kits. Random amplified polymorphic DNA analysis showed a higher discrimination power compared with the phenotypic analysis. The test strains were loosely clustered into six genotypic groups with no definite relation to any clinical events. These results indicate that many genotypes of Acinetobacter can cause adverse responses, and the initial source of the microorganisms rather than their clinical sequelae may determine classification.

Keywords: Acinetobacter; contact lenses; genotype; phenotype; random amplified polymorphic DNA analysis

Document Type: Research article

DOI: 10.1046/j.1442-9071.2001.00398.x

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