IngentaConnect Antibodies directed against ribosomal P proteins cross-react with...

Authors: Caponi, L.; Anzilotti, C.¹; Longombardo, G.¹; Migliorini, P.¹

Source: Clinical & Experimental Immunology, Volume 150, Number 1, October 2007 , pp. 140-143(4)

Abstract:

Summary

Anti-ribosomal P protein (anti-P) antibodies are marker antibodies in systemic lupus erythematosus (SLE). Their association with psychiatric or neurological manifestations has been proposed, but remains controversial. Anti-phospholipid antibodies are the hallmark of a syndrome that may comprise a number of neurological manifestations. Thus, anti-P and anti-phospholipid antibodies have both been associated with central nervous system involvement and their co-existence in the same sera was reported. We verified the ability of purified anti-P antibodies to bind different phospholipids and phospholipid-binding proteins in solid-phase assays. Anti-P antibodies from five of eight patients bound cardiolipin (CL) when saturated with fetal calf serum (FCS); in three cases anti-CL antibodies were also detected in the flow-through. No anti-P eluate, nor any corresponding flow-through, bound β₂-glycoprotein I alone or prothrombin. Moreover, no anti-P eluate bound CL when the plates were blocked with bovine serum albumin in the absence of FCS. Anti-P antibodies with anti-CL activity bound both ssDNA and dsDNA and also nucleosomes in three patients. Our data indicate a great heterogeneity of anti-P antibodies that appear to be overlapped partially with the other autoantibody populations detected frequently in SLE.

Document Type: Research article

DOI: 10.1111/j.1365-2249.2007.03466.x

Affiliations: 1: Department of Internal Medicine, University of Pisa, Via Roma 67 I-56126, Italy

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Antibodies directed against ribosomal P proteins cross-react with phospholipids