Heat shock proteins form part of a danger signal cascade in response to lipopolysaccharide and GroEL
Authors: Davies, E. L.1; Bacelar, M. M. F. V. G.1; Marshall, M. J.2; Johnson, E.3; Wardle, T. D.4; Andrew, S. M.1; Williams, J. H. H.
Source: Clinical & Experimental Immunology, Volume 145, Number 1, July 2006 , pp. 183-189(7)
Publisher: Blackwell Publishing
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Abstract:
Summary An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70 (0·22 and 0·7 ng/106 cells, respectively) into medium over 24 h at 37°C. Release of Hsp70 was stimulated 10-fold by GroEL (P < 0·001) and more than threefold by lipopolysaccharide (LPS) (P < 0·001). Although Hsp60 could be detected in the medium of cells cultured at 37°C for 24 h, the low rates of release were due probably to cell damage. Significant release of Hsp60 was observed when Jurkat cells were exposed to GroEL (2·88 ng/106 cells) or LPS (1·40 ng/106 cells). The data are consistent with the hypothesis that Hsp70 and Hsp60 are part of a danger signalling cascade in response to bacterial infection.Keywords: danger signals; GroEL; Hsp60 release; Hsp70 release; LPS; lymphocytes; peripheral blood mononuclear cells
Document Type: Research article
DOI: 10.1111/j.1365-2249.2006.03109.x
Affiliations: 1: Chester Centre for Stress Research, Biological Sciences, University of Chester, Chester, 2: Charles Salt Centre and 3: Spinal Studies, The Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, and 4: Countess of Chester Hospital Trust, Countess of Chester Health Park, Liverpool Road, Chester, UK
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