IngentaConnect Effects of alveolar macrophage depletion on liposomal vaccine pro...

Authors: Benoit, A.¹; Huang, Y.¹; Proctor, J.¹; Rowden, G.²; Anderson, R.

Source: Clinical & Experimental Immunology, Volume 145, Number 1, July 2006 , pp. 147-154(8)

Abstract:

Summary

Little is known about the identities and roles of antigen-presenting cells upon exposure to antigens of respiratory syncytial virus (RSV). Here, we focused on elucidating the importance of alveolar macrophages in conferring protective immunity in mice administered a liposome-encapsulated recombinant fragment of the RSV G protein. Mice were depleted of alveolar macrophages by intranasal inoculation of liposome-encapsulated dichloromethylenediphosphonic acid (DMDP). Mice depleted of alveolar macrophages prior to immunization developed reduced levels of serum RSV-neutralizing antibody and showed dramatically impaired protection against RSV challenge. The severity of interstitial inflammation was also markedly reduced in macrophage-depleted mice. In conclusion, this study demonstrates a pivotal role for alveolar macrophages during exposure to liposome-encapsulated RSV antigen in initiating both protective and histopathological responses against RSV.

Keywords: inflammation in RSV disease; respiratory syncytial viral G protein vaccine; vaccine-enhanced RSV disease

Document Type: Research article

DOI: 10.1111/j.1365-2249.2006.03114.x

Affiliations: 1: Departments of Microbiology and Immunology, 2: Pathology, and

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Effects of alveolar macrophage depletion on liposomal vaccine protection against respiratory syncytial virus (RSV)