IngentaConnect Immunostimulatory oligodeoxynucleotide from Bifidobacterium longu...

Authors: Takahashi, N.; Kitazawa, H.¹; Iwabuchi, N.²; Xiao, J. Z.²; Miyaji, K.²; Iwatsuki, K.²; Saito, T.¹

Source: Clinical & Experimental Immunology, Volume 145, Number 1, July 2006 , pp. 130-138(9)

Abstract:

Summary

We have reported previously that novel immunostimulatory sequence (ISS) oligodeoxynucleotide (ODN) BL07S from a probiotic strain of Bifidobacterium longum inhibited immunoglobulin (Ig) E production in vitro. However, whether ISS-ODNs from probiotics regulate T helper type 2 (Th2)-polarized immune reactions in vivo remains unclear. To evaluate the inhibitory effects of ODN BL07S on type I allergic response, BALB/c mice were injected with or without ODN BL07S in the presence of ovalbumin (OVA) on days 0 and 14. Serum Ig levels (IgE, IgG1 and IgG2a) and cytokine levels (interferon (IFN)-γ, interleukin (IL)-12, IL-4, IL-5, IL-10 and IL-13) were investigated in splenocyte cultures from days 14-28. Production of OVA-specific and total IgE were significantly suppressed by administration of ODN BL07S, but not by ODN BL06S, a non-ISS-ODN. Compared to controls, ODN BL07S induced significantly lower levels of Th2 cytokines (IL-4 and IL-5) in splenocyte cultures, and significantly higher levels of serum OVA-specific IgG2a. These effects of ODN BL07S on modulation of Th2 immune response were dose-dependent. The present results demonstrate that ODN BL07S from genomic DNA of B. longum BB536 prevents antigen-induced Th2 immune responses in vivo, suggesting that ISS-ODNs from probiotics might be useful in preventing allergic disease.

Keywords: allergy; Bifidobacterium longum BB536; IgE; ISS-ODN; probiotics

Document Type: Research article

DOI: 10.1111/j.1365-2249.2006.03111.x

Affiliations: 1: Graduate School of Agricultural Science, Tohoku University, Sendai, Japan 2: Food Research and Development Laboratory, Morinaga Milk Industry Co. Ltd, Zama, Japan, and

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Immunostimulatory oligodeoxynucleotide from Bifidobacterium longum suppresses Th2 immune responses in a murine model