Free Content Transfection of single-stranded hepatitis A virus RNA activates MHC class I pathway

Authors: SUZUKI, K.1; YANAGI, M.2; MORI-AOKI, A.1; MORIYAMA, E.1; ISHII, K. J.3; KOHN, L. D.1

Source: Clinical & Experimental Immunology, Volume 127, Number 2, February 2002 , pp. 234-242(9)

Publisher: Blackwell Publishing

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

SUMMARY

Although infection of single-stranded RNA viruses can enhance expression of major histocompatibility complex (MHC) class I genes, the mechanism underlying this process remains unclear. Recent studies have indicated that exposure of non-immune cells to double-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) of viral origin can directly increase the expression of MHC class I and related molecules without immune cell interaction. In this report, we show that transfection of single-stranded hepatitis A virus RNA into cultured hepatocytes results in the induction of genes for MHC class I, LMP2 and transporter for antigen processing (TAP1), in addition to the generation of viral proteins. We suggest that this stimulatory effect is due to the double-stranded RNA formed during replication of single-stranded viral RNA, and involves both double-stranded, RNA-dependent protein kinase PKR and the secretion of IFNβ.

Keywords: infectious immunity-virus; MHC; antigen presentation; protein kinases/phosphatases

Document Type: Research article

DOI: 10.1046/j.1365-2249.2002.01767.x

Affiliations: 1: Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Maryland, USA, 2: Department of Internal Medicine, Kanazawa University School of Medicine, Ishikawa, Japan, 3: Section of Retroviral Immunology, Center for Biologics, Evaluation, and Research, Food and Drug Administration, Maryland, USA, and

You have access to the full text article on a website external to Ingentaconnect.

Please click here to view this article on InterScience.

You may be required to register and activate access on InterScience before you can obtain the full text. If you have any queries please contact onlinehelp@oxon.blackwellpublishing.com

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A