IngentaConnect Eosinophil granule-derived major basic protein induces IL-8 expre...

Authors: Furuta, G. T.¹; Ackerman, S. J.²; Varga, J.³; Spiess, A. M.²; Wang, M. Y.¹; Wershil, B. K.⁴

Source: Clinical & Experimental Immunology, Volume 122, Number 1, October 2000 , pp. 35-40(6)

Abstract:

Eosinophil infiltration occurs in a variety of allergic and inflammatory diseases. The release of preformed mediators from eosinophils may contribute to inflammatory responses. We investigated the ability of eosinophil-derived major basic protein and eosinophil-derived neurotoxin to stimulate production of IL-8 from intestinal myofibroblasts. Intestinal myofibroblasts (18-Co cells) were incubated with major basic protein, eosinophil-derived neurotoxin, or a synthetic analogue of major basic protein, poly-l-arginine. Immunoreactive IL-8 was measured by ELISA and IL-8 mRNA levels were analysed by Northern blot or reverse transcription-polymerase chain assay. Major basic protein induced IL-8 mRNA production and release of significant levels of IL-8 immunoreactive protein. By contrast, eosinophil-derived neurotoxin stimulated little IL-8 release. The induction of IL-8 mRNA by poly-l-arginine was significantly inhibited by actinomycin D. These findings demonstrate a novel interaction between eosinophils and intestinal fibroblasts that may be involved in the pathogenesis of diseases associated with tissue eosinophilia.

Keywords: eosinophil; IL-8; major basic protein; gastrointestinal; fibroblast

Document Type: Research article

DOI: 10.1046/j.1365-2249.2000.01337.x

Affiliations: 1: Combined Program in Pediatric Gastroenterology and Nutrition, The Children's Hospital and Massachusetts General Hospital, and the Division of Experimental Pathology, Beth Israel Deaconess Medical Center, Boston, MA, 2: Department of Biochemistry and Molecular Biology and 3: Section of Rheumatology, Department of Medicine, College of Medicine, University of Illinois at Chicago, Chicago, IL, and 4: State University of New York Health Science Center at Brooklyn, Brooklyn, NY, USA

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Eosinophil granule-derived major basic protein induces IL-8 expression in human intestinal myofibroblasts