IngentaConnect IL-10 enhances IL-2-induced proliferation and cytotoxicity by hum...

Author: Ebert, E. C.¹

Source: Clinical & Experimental Immunology, Volume 119, Number 3, March 2000 , pp. 426-432(7)

Abstract:

IL-10 modulation of human intestinal T lymphocyte functions was studied for the first time. Lymphocyte proliferation was determined by ³H-thymidine incorporation; cytokine production, by ELISA; expression of surface markers, by immunofluorescence and flow cytometric analysis; and cytotoxicity, by lysis of ⁵¹Cr-labelled target cells. IL-10 blocked phytohaemagglutinin (PHA)-induced activation and proliferation of CD8⁺ T cells from the epithelium and lamina propria. It was a greater inhibitor of IL-2, interferon-gamma, and tumour necrosis factor-alpha production than were IL-4 or transforming growth factor-beta. In contrast, IL-10 enhanced IL-2-stimulated proliferation of both CD4⁺ and CD8⁺ T cells by increasing cell division after activation. It also augmented IL-2- but not IL-15-induced cytotoxicity of intestinal lymphocytes against colon cancer by a mechanism independent of natural killer cells. In conclusion, IL-10 blocking of proinflammatory cytokine secretion probably reduces intestinal inflammation. IL-10 augmentation of IL-2-induced cytotoxicity may help to maintain host defence.

Keywords: lymphokine-activated killer activity; tumour necrosis factor; interferon-gamma; IL-2; IL-10; inflammatory bowel disease

Document Type: Research article

DOI: 10.1046/j.1365-2249.2000.01147.x

Affiliations: 1: Department of Medicine, UMDNJ Robert Wood Johnson Medical School, New Brunswick, NJ, USA

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IL-10 enhances IL-2-induced proliferation and cytotoxicity by human intestinal lymphocytes