Identification of a peptide inducing experimental autoimmune uveoretinitis (EAU) in H-2Ak-carrying mice
Authors: Namba1; Ogasawara1; Kitaichi1; Matsuki1; Takahashi1; Sasamoto2; Kotake2; Matsuda2; Iwabuchi1; Ohno3; OnoÉ1
Source: Clinical & Experimental Immunology, Volume 111, Number 2, February 1998 , pp. 442-449(8)
Publisher: Blackwell Publishing
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Abstract:
When certain strains of mice bearing H-2Ak are immunized with the interphotoreceptor retinoid-binding protein (IRBP), EAU is induced. Thus far uveitogenic determinant(s) has not been determined in the H-2Ak mouse system. In addition it is hard to prepare purified IRBP. In the present study, to circumvent these problems we attempted to identify uveitogenic peptides derived from bovine IRBP in H-2Ak haplotype mice. Six peptides which had been selected according to the H-2Ak binding motif (Dxxxxxxxx[A, R, T]) were synthesized. We report here that all the peptides are immunogenic but only one peptide, K2, which consisted of IRBP201-216 residues, induces EAU in various mice carrying H-2Ak. Amino acid substitution of K2 revealed that the core region interacted with both H-2Ak and T cell antigen receptor (TCR). The amino acid sequence of the core region derived from bovine IRBP was identical to the corresponding region of mouse IRBP. In addition, K2 appeared to be a natural peptide antigen processed from bovine IRBP. Altogether, we concluded that K2 is one of the natural autoantigens involved in induction of EAU in H-2Ak mice.Keywords: peptide antigen; IRBP; EAU; MHC restriction
Document Type: Original article
DOI: 10.1046/j.1365-2249.1998.00514.x
Affiliations: 1: Section of Pathology, Institute of Immunological Science, 2: Department of Ophthalmology, School of Medicine, Hokkaido University, Sapporo, 3: Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
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