IngentaConnect A study of the epitopes on steroid 21-hydroxylase recognized by a...

Authors: Volpato¹; Prentice²; Chen²; Betterle¹; Rees Smith,²; Furmaniak²

Source: Clinical & Experimental Immunology, Volume 111, Number 2, February 1998 , pp. 422-428(7)

Abstract:

Steroid 21-hydroxylase (21-OH) autoantibodies are found in patients with autoimmune Addison's disease (AAD), either isolated or associated with autoimmune polyglandular syndrome (APS) type I and II and in adrenal-cortex autoantibody (ACA)-positive patients without AAD. In order to assess any differences in the 21-OH autoantibodies in these different patient groups, we have studied their reactivity with different epitopes on 21-OH using full length and modified ³⁵S-labelled 21-OH proteins produced in an in vitro transcription/translation system. There were no major differences in the pattern of autoantibody reactivity with the different modified 21-OH proteins in patients with isolated AAD or with APS types I and II, and in 21-OH autoantibody-positive patients with clinical AAD, subclinical AAD and those maintaining a normal adrenal function. Our studies also indicate that the main epitopes for 21-OH autoantibodies in patients with different forms of autoimmune adrenal disease are located in the C-terminal end and in a central region of 21-OH.

Keywords: 21-hydroxylase; autoantibodies; Addison's disease; 21-hydroxylase epitopes; polyglandular autoimmune syndromes

Document Type: Original article

DOI: 10.1046/j.1365-2249.1998.00475.x

Affiliations: 1: Istituto di Semeiotica Medica, University of Padova, Padova, Italy, 2: FIRS Laboratories, RSR Ltd.,

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A study of the epitopes on steroid 21-hydroxylase recognized by autoantibodies in patients with or without Addison's disease