Free Content Monocytes modulate enhancement of HIV-1 replication by Mycobacterium tuberculosis

Authors: DE Haas,1; DE Vos,1; Visser1; Snippe1; Verhoef1

Source: Clinical & Experimental Immunology, Volume 111, Number 2, February 1998 , pp. 286-292(7)

Publisher: Blackwell Publishing

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Abstract:

To investigate the effects of Mycobacterium tuberculosis on HIV-1 replication, peripheral blood mononuclear cells (PBMC) of bacille Calmette-Guérin (BCG)-vaccinated donors and non-BCG-vaccinated donors were infected in vitro with a lymphotropic isolate of HIV-1 and cultured in the presence of purified protein derivative (PPD). Addition of PPD resulted in enhanced HIV-1 replication and lymphoproliferation in BCG-vaccinated donor PBMC, while PPD had no such effects in control PBMC. HIV-1 replication increased even more when monocytes were removed from PBMC, while lymphoproliferation was decreased. High percentages of monocytes were associated with a decreased HIV-1 replication and proliferation that could not be reversed by addition of antibodies against the cytokines IL-1, transforming growth factor-beta (TGF-β) or indomethacin. PPD stimulates PBMC to release IL-10, a cytokine known to down-regulate proliferation and HIV-1 replication. PPD-induced effects on proliferation as well as HIV-1 replication could be partially blocked by adding a monoclonal antibody against MHC class II molecules, suggesting that part of the mechanism of PPD-induced enhancement is T memory cell activation.

Keywords: Mycobacterium; purified protein derivative; HIV; monocytes

Document Type: Original article

DOI: 10.1046/j.1365-2249.1998.00494.x

Affiliations: 1: Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, University Hospital, Utrecht, The Netherlands

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