IngentaConnect Isolation of a novel mouse gene, mSVS-1/SUSD2, reversing tumorige...

Authors: Sugahara, Tetsuo¹; Yamashita, Yzumi¹; Shinomi, Masahito¹; Yamanoha, Banri²; Iseki, Hiroyoshi³; Takeda, Akihiko³; Okazaki, Yasushi; Hayashizaki, Yoshihide⁴; Kawai, Kenji⁵; Suemizu, Hiroshi⁵; Andoh, Toshiwo

Source: Cancer Science, Volume 98, Number 6, June 2007 , pp. 900-908(9)

Publisher: Blackwell Publishing

Abstract:

We report isolation of a novel tumor-reversing gene, tentatively named SVS-1, encoding a protein of 820 amino acids with localization on the plasma membrane as a type I transmembrane protein. The gene was found among those downregulated in the activated oncogene-v-K-ras-transformed NIH3T3 cells, Ki3T3, with tumorigenic phenotype. SVS-1 protein harbors several functional domains inherent to adhesion molecules. Histochemical staining of mouse tissues using antibody raised against the protein showed the expression of the protein in restricted regions and cells, for example, strongly positive in apical membranes of epithelial cells in renal tubules and bronchial tubes. The protein inducibly expressed in human fibrosarcoma HT1080 cells and cervical carcinoma HeLa cells was found to be localized primarily on the plasma membrane, as stained with antibodies against FLAG tag in the N-terminus and against the C-terminal peptide of the protein. Expression of the protein in cells induced a variety of biological effects on cancer cells: detachment from the substratum and aggregation of cells and growth inhibition in HeLa cells, but no inhibition in non-tumorigenic mouse NIH3T3 cells. Inhibition of clonogenicity, anchorage-independent growth, migration and invasion through Matrigel was also observed. Taken together these results suggest that the SVS-1 gene is a possible tumor-reversing gene. (Cancer Sci 2007; 98: 900-908)

Document Type: Research article

DOI: 10.1111/j.1349-7006.2007.00466.x

Affiliations: 1: Department of Bioinformatics and 2: Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Hachioji, Tokyo 192-8577; 3: Department of Surgery and Surgical Oncology, Saitama Medical University, Iruma-gun, Saitama 350-0495; 4: Genomics Science Center, The Institute of Physical and Chemical Research, Konodai, Tsukuba-city 305-0074; 5: Biomedical Research Department, Central Institute for Experimental Animals, Miyamae-ku, Kawasaki 216-0001, Japan

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Isolation of a novel mouse gene, mSVS-1/SUSD2, reversing tumorigenic phenotypes of cancer cells in vitro