IngentaConnect Nitrative and oxidative DNA damage in oral lichen planus in relat...

Authors: Chaiyarit, Ponlatham¹; Ma, Ning²; Hiraku, Yusuke³; Pinlaor, Somchai; Yongvanit, Puangrat; Jintakanon, Darunee⁴; Murata, Mariko³; Oikawa, Shinji³; Kawanishi, Shosuke

Source: Cancer Science, Volume 96, Number 9, September 2005 , pp. 553-559(7)

Publisher: Blackwell Publishing

Abstract:

Oral lichen planus (OLP) is a chronic inflammatory disease, which has been clinically associated with development to oral cancer. A double immunofluorescence labeling study found that 8-nitroguanine and 8-oxo-7,8-dihydro-2 prime

-deoxyguanosine (8-oxodG) accumulated in oral epithelium in OLP and oral squamous cell carcinoma (OSCC) biopsy specimens, whereas little or no immunoreactivity was observed in normal oral mucosa. Colocalization of 8-nitroguanine and inducible nitric oxide synthase (iNOS) was found in oral epithelium of OLP and OSCC. Immunoreactivity of 3-nitrotyrosine, which is formed by protein tyrosine nitration and is considered to be a biochemical marker for inflammation, was also observed in oral epithelial cells and colocalized with 8-nitroguanine. Accumulation of p53 was more strongly observed in oral epithelium in OSCC than OLP, whereas there was no p53 accumulation in normal oral mucosa. Our findings demonstrate that iNOS-dependent DNA damage in OLP may lead to p53 accumulation in not only OLP but also OSCC. We conclude that the formation of potentially mutagenic DNA lesions including 8-nitroguanine and 8-oxodG may contribute to the development of oral cancer from OLP. (Cancer Sci 2005; 96: 553 –559)

Document Type: Research article

DOI: 10.1111/j.1349-7006.2005.00096.x

Affiliations: 1: Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand; 2: Department of Anatomy and 3: Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Mie 514-8507, Japan; and 4: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

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Nitrative and oxidative DNA damage in oral lichen planus in relation to human oral carcinogenesis