IngentaConnect Cloning of novel LERGU mRNAs in GPR30 3 untranslated region and d...

Authors: Kakinuma, Naoto¹; Sato, Masaaki¹; Yamada, Tatsuya²; Kohu, Kazuyoshi; Nakajima, Motowo¹; Akiyama, Tetsu³; Ohwada, Susumu²; Shibanaka, Yasuhiko

Source: Cancer Science, Volume 96, Number 3, March 2005 , pp. 191-196(6)

Publisher: Blackwell Publishing

Abstract:

The improved IGCR (In–Gel Competitive Reassociation) method was applied to the analysis of human gastric cancer genomic DNA to identify its alterations, and it appeared that the IGCR library contained a fragment of 3 prime

-untranslated region (3 prime

UTR) of G-protein coupled receptor 30 (GPR30) mRNA. When we searched genomic DNA pairs of gastric cancer patients with this IGCR clone, we found the deletion polymorphism with or without 2 bp (Cytosine and Thymine; CT). We confirmed the existence of a novel mRNA in GPR30 3 prime

UTR by northern blotting, cloned this novel mRNA and named it Leucine Rich Protein in GPR30 3 prime

UTR (LERGU). The EST database search gave one alternative splicing form in this 3 prime

UTR, which was named as LERGU-1. A novel alternative splicing form of this mRNA was also identified from the stomach total RNA, which was named LERGU-2. The LERGU mRNA was also detected in eight gastric cancer cell lines, but GPR30 mRNA scarcely existed. Furthermore, we detected the 2 bp-deletion form in genomic DNAs and mRNAs derived from gastric cancers, but not in other type cancers. Since the 2 bp-deletion position on LERGU corresponds to its alternative splicing site, this deletion may produce a frame-shifted protein. Overall, our findings suggest that a mutation or disappearance of the normal LERGU protein may have a function in the development of gastric cancer. (Cancer Sci 2005; 96: 191–196)

Document Type: Research article

DOI: 10.1111/j.1349-7006.2005.00031.x

Affiliations: 1: Tsukuba Research Institute, Novartis Pharma K.K., Ohkubo 8, Tsukuba-shi Ibaraki 300-2611; 2: Second Department of Surgery, Gunma University Faculty of Medicine, 3-39-15 Showa-Machi, Maebashi, Gunma 371; and 3: Laboratory of Molecular and Genetic Information, Institute of Molecular and Biological Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan

You have access to the full text article on a website external to Ingentaconnect.

Please click here to view this article on InterScience.

You may be required to register and activate access on InterScience before you can obtain the full text. If you have any queries please contact onlinehelp@oxon.blackwellpublishing.com

Cloning of novel LERGU mRNAs in GPR30 3 untranslated region and detection of 2 bp-deletion polymorphism in gastric cancer