IngentaConnect Helicobacter pylori-dependent NF-kappa B activation in newly esta...

Authors: Nozaki, Koji; Tanaka, Harunari¹; Ikehara, Yuzuru; Cao, Xueyuan; Nakanishi, Hayao¹; Azuma, Takeshi²; Yamazaki, Shiho²; Yamaoka, Yoshio³; Shimizu, Nobuyuki⁴; Mafune, Ken-ichi⁴; Kaminishi, Michio⁴; Tatematsu, Masae¹

Source: Cancer Science, Volume 96, Number 3, March 2005 , pp. 170-175(6)

Publisher: Blackwell Publishing

Abstract:

Mongolian gerbils are an ideal animal model to explore the role of H. pylori on cancer development. However, there have been no established adenocarcinoma cell lines from this model animal. In the present study, we have established cancer cell lines from a primary gastric cancer tissue of a Mongolian gerbil. The derived cells could be stably attached with H. pylori, revealed under a scanning electron microscope, and easily transplanted to the nude mice. Rapid phosphorylation of I kgr

B, Erk1/2, and AKT of these cells was observed by Interleukin-1 beta stimulation, and luciferase reporter gene assay on transcriptional activation of Nuclear Factor kappa B after challenging with either H. pylori NCTC11637 or its isogenic cagE-knockout mutant, H. pylori revealed the cagE-dependent NF- kgr

B transcriptional activation. The newly established cancer cell lines from the in vivo gastric carcinogenesis model animal, the Mongolian gerbil, can be used to develop effective therapeutic strategies against gastric cancer, especially in exploring the effect of H. pylori, and thus might greatly contribute to gastric cancer prevention and treatment in humans. (Cancer Sci 2005; 96: 170–175)

Document Type: Research article

DOI: 10.1111/j.1349-7006.2005.00030.x

Affiliations: 1: Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya, Aichi 464-8681; 2: Second Department of Internal Medicine, Faculty of Medical Science, University of Fukui, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan; and 3: Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas 77030, USA 4: Department of Gastrointestinal Surgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655;

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Helicobacter pylori-dependent NF-kappa B activation in newly established Mongolian gerbil gastric cancer cell lines