Tracking and characterisation of transfused platelets by two colour, whole blood flow cytometry

Authors: Hughes, Darren L.1; Evans, Gillian2; Metcalfe, Paul3; Goodall, Alison H.4; Williamson, Lorna M.

Source: British Journal of Haematology, Volume 130, Number 5, September 2005 , pp. 791-794(4)

Publisher: Blackwell Publishing

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Abstract:

Summary

We describe a flow cytometric technique to study transfused platelets in patients. By selecting donor/recipient pairs discrepant for HLA-A2, transfused platelets were identified using anti-HLA-A2 with a detection limit of <5%, and accuracy within 4·3% of predicted (r2 > 0·96, P < 0·0001). In two of three episodes, transfused platelets were present in greater numbers than predicted from increment counts. Platelets with bound fibrinogen fell from 20·9 ± 23·6% of donor platelets pretransfusion to 1·7 ± 0·3% 1 h post-transfusion, whereas P-selectin-positive platelets fell gradually, from 24·1 ± 6·7 to 7·3 ± 3·3% at 6 h. This method avoids radio or chemical labelling, and could be used to assess novel platelet preparations post-transfusion.

Keywords: platelets; transfusion; flow cytometry; platelet activation

Document Type: Research article

DOI: 10.1111/j.1365-2141.2005.05671.x

Affiliations: 1: Department of Haematology, University of Cambridge 2: Department of Haematology, Addenbrooke's Hospital, Cambridge 3: Haematology Division, National Institute for Biological Standards and Control, Potters Bar 4: Department of Cardiovascular Sciences, University of Leicester, Leicester

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