Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro

Authors: Head, David J.; Lee, Zoe E.; Swallah, Muhammed M.; Avent, Neil D.

Source: British Journal of Haematology, Volume 130, Number 5, September 2005 , pp. 788-790(3)

Publisher: Blackwell Publishing

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Abstract:

Summary

CD47 ligation has been shown to induce phosphatidylserine (PS) expression as part of a death pathway in nucleated blood cells. Using Annexin V binding assays we showed that ligation of CD47 with the specific CD47-binding peptide 41NK, anti-CD47 monoclonal antibody, and its natural ligand thrombospondin-1 also induced PS expression on enucleated erythrocytes. PS expression was associated with a concomitant loss of erythrocyte viability in vitro. Further characterisation of the CD47-PS signalling pathway on erythrocytes may help develop clinical strategies to further preserve the life of blood donations and improve our understanding of certain types of haemolytic anaemias.

Keywords: CD47; erythrocytes; phosphatidylserine; thrombospondin-1; viability

Document Type: Research article

DOI: 10.1111/j.1365-2141.2005.05668.x

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