Progenitor cell subsets and engraftment kinetics in children undergoing autologous peripheral blood stem cell transplantation

Authors: Gonzalez-Requejo, Angel1; Madero, Luis2; DÍaz, Miguel A.2; Villa, Marta2; GarcÍA-Escribano, Carmen1; Balas, Antonio1; Lillo, Rosa1; GarcÍA-Sanchez, Félix1; Benito, Ana2; Vicario, Jose L.1

Source: British Journal of Haematology, Volume 101, Number 1, April 1998 , pp. 104-110(7)

Publisher: Blackwell Publishing

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Abstract:

The main objective of the present study was to determine the role of CD34+ cell subsets in the haemopoietic recovery of children undergoing peripheral blood stem cell transplantation. For this purpose, 38 leukaphereses from 33 children with malignancies mobilized with G-CSF were analysed. Using dual-colour flow cytometry, different subpopulations of CD34+ cells were quantified and the number of each reinfused subsets correlated with haemopoietic resurgence. Multivariate analysis showed that the number of CD34+CD38− cells and CD34+CD38+ cells correlated better with time to neutrophil and platelet recovery, respectively, than the total number of CD34+ cells. Threshold values for rapid haemopoietic recovery, determined by the receiver operating characteristic analysis, were found to be 0.5 × 106 CD34+CD38− cells for neutrophil engraftment, and 2.0 × 106 CD34+CD38+ cells for platelet recovery. It is suggested that the analysis of CD34+ cell subsets could increase understanding of the repopulation capacity of a given leukapheresis product in peripheral blood stem cell transplantation procedures in children. In particular, this procedure could be extremely useful when low numbers of CD34+ cells are collected.

Keywords: peripheral blood stem cell transplantation; CD34+ cell subsets; flow cytometry; haemopoietic recovery; children

Document Type: Original article

DOI: 10.1046/j.1365-2141.1998.00662.x

Affiliations: 1: Histocompatibility, Regional Transfusion Centre, Madrid, 2: Oncology and Bone Marrow Transplantation Unit, `Niño Jesús' Children Hospital, Madrid, Spain

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