Rapid heterozygote detection in Glanzmann's thrombasthenia

Authors: Sharp¹; Khanduri¹; Christie¹

Source: British Journal of Haematology, Volume 101, Number 1, April 1998 , pp. 66-69(4)

Publisher: Blackwell Publishing

Key:

- Free Content

- New Content

- Subscribed Content

- Free Trial Content

Abstract:

To assess the potential of using flow cytometric analysis of platelet glycoprotein IIb in the detection of heterozygotes in Glanzmann's thrombasthenia (GT) we compared the fluorescence intensity of anti-CD41-labelled platelets of obligatory heterozygotes with that of normal subjects. This study showed that normal subjects had relative fluorescences occasionally overlapping with heterozygotes. Use of an independent factor, the mean platelet volume, as a correction factor for platelet size differences eliminated this overlap. This study indicates that it is possible to confidently and rapidly predict the carrier status of family members of GT sufferers, both type I and type II, by flow cytometric means.

Keywords: thrombasthenia; CD41; MPV; flow cytometry; whole blood lysis

Document Type: Research article

DOI: 10.1046/j.1365-2141.1998.00655.x

Affiliations: 1: Department of Haematology, College of Medicine, Sultan Qaboos University, Oman

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy