IngentaConnect Biphasic expression of stromal cell-derived factor-1 during human...

Authors: Toksoy, A.¹; Müller, V.; Gillitzer, R.¹; Goebeler, M.

Source: British Journal of Dermatology, Volume 157, Number 6, December 2007 , pp. 1148-1154(7)

Abstract:

Summary Background

Chemokines tightly regulate the spatial and temporal infiltration of invading leucocyte subsets during wound healing. Stromal cell-derived factor-1 (SDF-1/CXCL12) is a homeostatic chemokine with multiple functions; its role during cutaneous wound healing, however, needs to be explored. Objectives

To elucidate expression of the multifunctional CXC chemokine SDF-1/CXCL12 during human wound healing. Methods

Skin biopsies were obtained from 14 volunteers between 1 and 21 days after incisional wounding and processed for in situ hybridization and immunohistochemistry. Results

We analysed the spatial and temporal distribution of SDF-1/CXCL12 after artificial wounding and detected a complete downregulation at both the mRNA and the protein level within the fibrous stroma that replaces the initial wound defect. However, increased levels of SDF-1/CXCL12 were observed at the wound margins. Focusing on mediators regulating SDF-1/CXCL12 expression in vitro we realized that both tumour necrosis factor-α and interferon-γ downregulated its expression in human dermal microvascular endothelial cells and fibroblasts. Conclusions

Our data suggest that SDF-1/CXCL12 is tightly regulated during wound repair. Increased expression at the wound margin may contribute to the accumulation of endothelial progenitor cells, thus accelerating neovascularization.

Keywords: chemokines; CXCL12; CXCR4; endothelial cells; stromal cell-derived factor-1; wound healing

Document Type: Research article

DOI: 10.1111/j.1365-2133.2007.08240.x

Affiliations: 1: Department of Dermatology, University of Würzburg, Würzburg, Germany

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.16 plus tax Refund Policy

Biphasic expression of stromal cell-derived factor-1 during human wound healing