IngentaConnect Interleukin-19 upregulates keratinocyte growth factor and is asso...

Authors: Li, Hsing-Hui; Lin, Y-C.¹; Chen, P-J.²; Hsiao, C-H.³; Lee, J.Y-Y.⁴; Chen, W-C.⁵; Tzung, T-Y.⁶; Wu, J-C.⁶; Chang, Ming-Shi

Source: British Journal of Dermatology, Volume 153, Number 3, September 2005 , pp. 591-595(5)

Publisher: Blackwell Publishing

Abstract:

Summary Background

Interleukin (IL)-19, a member of the IL-10 family, signals through the IL-20R1/IL-20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis. Objectives

To investigate the role of IL-19 in the psoriatic process. Methods

The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL-19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL-19 in the sera of patients with psoriasis and healthy volunteers using an enzyme-linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls. Results

We found that IL-19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL-19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0·05). IL-19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis. Conclusions

These results suggest that IL-19 plays a role in the complex pathological cytokine network in psoriasis.

Keywords: interleukin-19; keratinocyte growth factor; keratinocytes; psoriasis

Document Type: Research article

DOI: 10.1111/j.1365-2133.2005.06665.x

Affiliations: 1: Department of Dermatology, Chi Mei Medical Center, Tainan, Taiwan 2: Graduate Institute of Biochemistry 3: Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan 4: Department of Dermatology, Medical College, National Cheng Kung University, no. 1 Ta-Hsueh Road, Tainan 704, Taiwan 5: Department of Dermatology, Chang Gung Memorial Hospital, Kaohsiung, 6: Department of Dermatology, Veteran General Hospital, Kaohsiung, Taiwan

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Interleukin-19 upregulates keratinocyte growth factor and is associated with psoriasis