Crystallization and preliminary crystallographic studies of the D59A mutant of MicA, a YycF response-regulator homologue from Streptococcus pneumoniae

Authors: Riboldi-Tunnicliffe, Alan; Trombe, Marie-Claude; Bent, Colin J.; Isaacs, Neil W.; Mitchell, Timothy J.

Source: Acta Crystallographica Section D, Volume 60, Number 5, May 2004 , pp. 950-951(2)

Publisher: Blackwell Publishing

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Abstract:

RR02 (MicA) is an essential bacterial protein that belongs to the YycF family of response regulators and consists of two domains: an N-terminal receiver domain and a C-terminal effector domain. Streptococcus pneumoniae RR02 (MicA; residues 2-234) has been crystallized using the sitting-drop vapour-diffusion technique. The crystals belong to space group P2₁, with unit-cell parameters a = 46.46, b = 32.61, c = 63.35 Å, β = 90.01°. X-ray diffraction data have been collected to 1.93 Å resolution.

Keywords: S. pneumoniae; YycF homologue; response regulator; OmpR

Document Type: Research article

DOI: 10.1107/S0907444904005712

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